| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Ligilactobacillus ruminis | TGGAATTGCGCAATCCGAC | ACTGTGCAACGCCTTCCA | 59.20 | 59.81 | 273 | 135 |
100.00%
|
50.00%
|
| Primer 22 | Ligilactobacillus ruminis | TGACACGGCAATGGTGGA | ATTCAGGCCAGTAGCACCG | 59.17 | 59.78 | 196 | 135 |
100.00%
|
50.00%
|
| Primer 23 | Ligilactobacillus ruminis | TTTTGCAAAGCGGTGCCG | TTGTTTCGTCCCGTCGCA | 60.28 | 59.89 | 272 | 135 |
100.00%
|
50.00%
|
| Primer 24 | Ligilactobacillus ruminis | AATCCGACTTGTGGGGACG | ACTGTGCAACGCCTTCCA | 59.70 | 59.81 | 262 | 132 |
100.00%
|
50.00%
|
| Primer 25 | Ligilactobacillus ruminis | TTTTGATGTCGGCAATCGTTG | CTCCAAACGCGAATTGCTCA | 58.07 | 59.48 | 188 | 132 |
100.00%
|
50.00%
|
| Primer 26 | Ligilactobacillus ruminis | GGCAAACGAGTGGCTTTGG | AACACGGCTGCCAAAAGC | 60.01 | 59.58 | 171 | 132 |
100.00%
|
50.00%
|
| Primer 27 | Ligilactobacillus ruminis | TCGCATCCAGCCGTTTCA | ACGTCTGAACCAGCGCAT | 59.65 | 59.66 | 279 | 132 |
100.00%
|
50.00%
|
| Primer 28 | Ligilactobacillus ruminis | TCGGCTTTGCGATGCTGA | AGCAGAACCAGCGATGTGA | 60.05 | 59.33 | 232 | 132 |
100.00%
|
50.00%
|
| Primer 29 | Ligilactobacillus ruminis | TCGGCTTTGCGATGCTGA | TCAGCAGAACCAGCGATGT | 60.05 | 59.33 | 234 | 132 |
100.00%
|
50.00%
|
| Primer 30 | Ligilactobacillus ruminis | TCGGCTTTGCGATGCTGA | CAGCAGAACCAGCGATGTG | 60.05 | 59.21 | 233 | 132 |
100.00%
|
50.00%
|